SAGE Library SM249

Same tissue sample as SM225.

Classification

Library Name SM249
Species Mus musculus
Strain CBA/B6 (n=2 on B6)
Mutation NGN3-GFP Tg
Location Pancreas – NGN3-GFP+
Sex M and F
Age E13.5
Developmental Stage Theiler Stage 21
Organsim Condition Healthy

Sample Collection

Tissue Dissection Photos

Whole pancreas was dissected out of the embryos and a single cell suspension was created which was subjected to FACSorting to isolate the GFP+ cell population. This population was used for library construction. Includes all endocrine progenitor cells as well as endocrine cells at various stages of maturation

Lab Cheryl Helgason: BCCRC
Number of Animals 64

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 36.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 229,722
Total Raw Tags at Q95 192,661
Total Raw Tags at Q99 181,696
Unique Tags 62,257

Data

Tag Counts at Quality 0.95 cut-off tagcounts_SM249_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM249_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM249_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.