SAGE Library SM249
Same tissue sample as SM225.
Classification
| Library Name |
SM249 |
| Species |
Mus musculus |
| Strain |
CBA/B6 (n=2 on B6) |
| Mutation |
NGN3-GFP Tg |
| Location |
Pancreas – NGN3-GFP+ |
| Sex |
M and F |
| Age |
E13.5 |
| Developmental Stage |
Theiler Stage 21 |
| Organsim Condition |
Healthy |
Sample Collection
| Tissue Dissection Photos |
Whole pancreas was dissected out of the embryos and a single cell suspension was created which was subjected to FACSorting to isolate the GFP+ cell population. This population was used for library construction. Includes all endocrine progenitor cells as well as endocrine cells at various stages of maturation
|
| Lab |
Cheryl Helgason: BCCRC |
| Number of Animals |
64 |
Library Construction
| SAGE Protocol |
SAGE (LongSAGE 21 bp) |
| Starting RNA |
36.0 ng
|
|
Tag Extraction
| Total Raw Tags (no quality cut-off) |
229,722 |
| Total Raw Tags at Q95 |
192,661 |
| Total Raw Tags at Q99 |
181,696 |
| Unique Tags |
62,257 |
Data
Data Analysis
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Data is available for analysis using DiscoverySpace,
a cross-platform application that allows comparison between SAGE libraries,
tag to gene mapping and results from public datasources.
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SAGE Library Help
A full explanation of each of the fields displayed on this page is available on the
SAGE Library Help page.
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