SAGE Library SM246

Same tissue sample as SM206.

Classification

Library Name SM246
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Heart - Atrioventricular canal
Sex Unknown
Age E9.5
Developmental Stage Theiler Stage 15
Organsim Condition

Sample Collection

Tissue Dissection Photos

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With the aid of a dissecting microscope hearts were manually removed from embryos submerged in phosphate buffered saline (PBS). The atrio-ventricular canal was isolated using 30G1/2 needles. Tissue was immediately placed in Trizol. Multiple litters were used to extract RNA.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab BCCRC: Pamela Hoodless Lab
Number of Animals 79

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 550.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 207,519
Total Raw Tags at Q95 166,507
Total Raw Tags at Q99 158,105
Unique Tags 48,337

Data

Tag Counts at Quality 0.95 cut-off tagcounts_SM246_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM246_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM246_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.