SAGE Library SM245
Same tissue sample as SM162.
Classification
| Library Name |
SM245 |
| Species |
Mus musculus |
| Strain |
CBA/B6 (n=2 on B6) |
| Mutation |
NGN3-GFP Tg |
| Location |
Pancreas - NGN3-GFP- |
| Sex |
M and F |
| Age |
E12.5 |
| Developmental Stage |
Theiler Stage 20 |
| Organsim Condition |
Healthy |
Sample Collection
| Tissue Dissection Notes |
Whole pancreas was dissected out of the embryos and a single cell suspension was created which was subjected to FACSorting to isolate the GFP- cell population. This population was used for library construction. Includes a mixture of pancreas cell types composed predominantly of mesenchymal cells and pancreas epithelial progenitors as well as those destined to become exocrine cell types.
|
| Lab |
Cheryl Helgason: BCCRC |
| Number of Animals |
64 |
Library Construction
| SAGE Protocol |
SAGELite (LongSAGE 21 bp) |
| Starting RNA |
20 ng
|
|
Tag Extraction
| Total Raw Tags (no quality cut-off) |
199,717 |
| Total Raw Tags at Q95 |
161,262 |
| Total Raw Tags at Q99 |
151,987 |
| Unique Tags |
52,490 |
Data
Data Analysis
|
Data is available for analysis using DiscoverySpace,
a cross-platform application that allows comparison between SAGE libraries,
tag to gene mapping and results from public datasources.
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SAGE Library Help
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