SAGE Library SM244

Same tissue sample as SM161.

Classification

Library Name SM244
Species Mus musculus
Strain CBA/B6 (n=2 on B6)
Mutation PDX1-GFP Tg
Location Pancreas - PDX1-GFP+
Sex M and F
Age E10.5
Developmental Stage Theiler Stage 17
Organsim Condition Healthy

Sample Collection

Tissue Dissection Notes

Whole pancreas was dissected out of the embryos and a single cell suspension was created which was subjected to FACSorting to isolate the GFP+ cell population. This population was used for library construction. Includes all pancreas epithelial cells with the exception of the rare glucagon positive cells.

Lab Cheryl Helgason: BCCRC
Number of Animals 48

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 20.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 201,472
Total Raw Tags at Q95 151,302
Total Raw Tags at Q99 141,460
Unique Tags 55,332

Data

Tag Counts at Quality 0.95 cut-off tagcounts_SM244_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM244_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM244_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.