SAGE Library SM244
Same tissue sample as SM161.
Classification
| Library Name |
SM244 |
| Species |
Mus musculus |
| Strain |
CBA/B6 (n=2 on B6) |
| Mutation |
PDX1-GFP Tg |
| Location |
Pancreas - PDX1-GFP+ |
| Sex |
M and F |
| Age |
E10.5 |
| Developmental Stage |
Theiler Stage 17 |
| Organsim Condition |
Healthy |
Sample Collection
| Tissue Dissection Notes |
Whole pancreas was dissected out of the embryos and a single cell suspension was created which was subjected to FACSorting to isolate the GFP+ cell population. This population was used for library construction. Includes all pancreas epithelial cells with the exception of the rare glucagon positive cells.
|
| Lab |
Cheryl Helgason: BCCRC |
| Number of Animals |
48 |
Library Construction
| SAGE Protocol |
SAGELite (LongSAGE 21 bp) |
| Starting RNA |
20.0 ng
|
|
Tag Extraction
| Total Raw Tags (no quality cut-off) |
201,472 |
| Total Raw Tags at Q95 |
151,302 |
| Total Raw Tags at Q99 |
141,460 |
| Unique Tags |
55,332 |
Data
Data Analysis
|
Data is available for analysis using DiscoverySpace,
a cross-platform application that allows comparison between SAGE libraries,
tag to gene mapping and results from public datasources.
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SAGE Library Help
A full explanation of each of the fields displayed on this page is available on the
SAGE Library Help page.
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