SAGE Library SM243

Same tissue sample as SM160.

Classification

Library Name SM243
Species Mus musculus
Strain CBA/B6 (n=2 on B6)
Mutation NGN3-GFP Tg
Location Pancreas - NGN3-GFP+
Sex M and F
Age E12.5
Developmental Stage Theiler Stage 20
Organsim Condition Healthy

Sample Collection

Tissue Dissection Notes

Whole pancreas was dissected out of the embryos and a single cell suspension was created which was subjected to FACSorting to isolate the GFP+ cell population. This population was used for library construction. Includes all endocrine progenitor cells as well as endocrine cells at various stages of maturation.

Lab Cheryl Helgason: BCCRC
Number of Animals 64

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 20 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 222,272
Total Raw Tags at Q95 174,950
Total Raw Tags at Q99 165,141
Unique Tags 59,529

Data

Tag Counts at Quality 0.95 cut-off tagcounts_SM243_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM243_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM243_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.