SAGE Library SM235

Classification

Library Name SM235
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Heart - Outflow Tract
Sex Unknown
Age E10.5
Developmental Stage Theiler Stage 17
Organsim Condition

Sample Collection

Tissue Dissection Photos

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With the aid of a dissecting microscope hearts were manually removed from embryos submerged in phosphate buffered saline (PBS). The out-flow tract region was isolated using fine forceps. The region designated out-flow tract is shown in the organogenic image. As much blood was removed as possible before transfer to Trizol. The out-flow tract of embryos of several litters were collected to obtain enough RNA for SAGE library construction.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab Hoodless/Karsan: BCCRC
Number of Animals 25

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 2500.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 313,656
Total Raw Tags at Q95 254,612
Total Raw Tags at Q99 240,580
Unique Tags 68,455

Data

Tag Counts at Quality 0.95 cut-off tagcounts_SM235_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM235_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM235_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.