SAGE Library SM233

Classification

Library Name SM233
Species Mus musculus
Strain CBA/B6 (n=2 on B6)
Mutation PDX1-GFP Tg
Location Pancreas - PDX1-GFP+
Sex M and F
Age E9.5
Developmental Stage Theiler Stage 15
Organsim Condition Healthy

Sample Collection

Tissue Dissection Notes

Whole pancreas was dissected out of the embryos and a single cell suspension was created which was subjected to FACSorting to isolate the GFP+ cell population. This population was used for library construction.

Lab Cheryl Helgason: BCCRC
Number of Animals 48

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 28.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 301,688
Total Raw Tags at Q95 231,570
Total Raw Tags at Q99 216,914
Unique Tags 72,539

Data

Tag Counts at Quality 0.95 cut-off tagcounts_SM233_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM233_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM233_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

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