Classification
| Library Name |
SM233 |
| Species |
Mus musculus |
| Strain |
CBA/B6 (n=2 on B6) |
| Mutation |
PDX1-GFP Tg |
| Location |
Pancreas - PDX1-GFP+ |
| Sex |
M and F |
| Age |
E9.5 |
| Developmental Stage |
Theiler Stage 15 |
| Organsim Condition |
Healthy |
Sample Collection
| Tissue Dissection Notes |
Whole pancreas was dissected out of the embryos and a single cell suspension was created which was subjected to FACSorting to isolate the GFP+ cell population. This population was used for library construction.
|
| Lab |
Cheryl Helgason: BCCRC |
| Number of Animals |
48 |
Library Construction
| SAGE Protocol |
SAGELite (LongSAGE 21 bp) |
| Starting RNA |
28.0 ng
|
|
Tag Extraction
| Total Raw Tags (no quality cut-off) |
301,688 |
| Total Raw Tags at Q95 |
231,570 |
| Total Raw Tags at Q99 |
216,914 |
| Unique Tags |
72,539 |
Data
Data Analysis
|
Data is available for analysis using DiscoverySpace,
a cross-platform application that allows comparison between SAGE libraries,
tag to gene mapping and results from public datasources.
|
SAGE Library Help
A full explanation of each of the fields displayed on this page is available on the
SAGE Library Help page.
|