SAGE Library SM231

Classification

Library Name SM231
Species Mus musculus
Strain CBA/B6 (n=2 on B6)
Mutation PDX1-GFP Tg
Location Pancreas - PDX1-GFP+
Sex M and F
Age E11.5
Developmental Stage Theiler Stage 19
Organsim Condition Healthy

Sample Collection

Tissue Dissection Notes

Whole pancreas was dissected out of the embryos and a single cell suspension was created which was subjected to FACSorting to isolate the GFP+ cell population. This population was used for library construction. Includes all pancreas epithelial cells with the exception of the rare glucagon positive cells.

Lab Cheryl Helgason: BCCRC
Number of Animals 48

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 20 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 317,716
Total Raw Tags at Q95 244,088
Total Raw Tags at Q99 230,161
Unique Tags 82,085

Data

Tag Counts at Quality 0.95 cut-off tagcounts_SM231_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM231_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM231_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.