SAGE Library SM197

Classification

Library Name SM197
Species Mus musculus
Strain C57BL/6J
Mutation Fierce
Location Brain - VZ/SVZ dorsal telencephalon
Sex Male
Age E13.5
Developmental Stage Theiler Stage 21
Organsim Condition

Sample Collection

Tissue Dissection Photos

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An intact sagittal cryosection of brain of mouse at E13.5 stained with cresyl violet. LV = lateral ventricle; Str = striatum; VZ/SVZ = ventricular/subventricular zone. Scale bar = 100 m.


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The VZ/SVZ of dorsal telencephalon was cut with laser. Scale bar = 100 m.


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The VZ/SVZ of dorsal telencephalon was removed by LCM. Scale bar = 100 m.


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The VZ/SVZ of dorsal telencephalon was captured by LCM for total RNA extraction. Scale bar = 100 m.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals Not Available

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 20.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 110,030
Total Raw Tags at Q95 82,214
Total Raw Tags at Q99 79,484
Unique Tags 30,786

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM197_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM197_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM197_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.