SAGE Library SM196

Classification

Library Name SM196
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - Cerebellum, purkinje cell layer
Sex Male
Age P84-P90
Developmental Stage 12 weeks post birth
Organsim Condition

Sample Collection

Tissue Dissection Photos

Click to view full-size image…

An intact sagittal cryosection of mouse brain at postnatal week 12 stained with cresyl violet. M = molecular cell layer; P = purkinje cell layer; G = granule cell layer; WM = white matter. Scale bar = 200 µm.


Click to view full-size image…

An image of the cerebellum at higher amplification. M, molecular cell layer; P, purkinje cell layer; G, granule cell layer; WM, white matter. Scale bar = 200 µm.


Click to view full-size image…

The purkinje cell layer was cut with laser. Scale bar = 200 µm.


Click to view full-size image…

The purkinje cell layer was removed by LCM . Scale bar = 200 µm.


Click to view full-size image…

The purkinje cell layer was captured by LCM for total RNA extraction. Scale bar = 200 µm.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals Not Available

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 18.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 104,920
Total Raw Tags at Q95 81,434
Total Raw Tags at Q99 77,296
Unique Tags 37,392

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM196_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM196_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM196_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.