SAGE Library SM186

Classification

Library Name	SM186
Species	Mus musculus
Strain	C57BL/6J
Mutation	Wild Type
Location	Brain - CA1, dorsal/anterior
Sex	Male
Age	P35
Developmental Stage	Post natal day 35
Organsim Condition

Sample Collection

Tissue Dissection Photos	Click to view full-size image… An intact coronal cryosection of mouse brain at postnatal day 35 stained with cresyl violet. Ctx = cerebral cortex; cc = corpus callosum; DG = dentate gyrus. Scale bar = 200 µm. Click to view full-size image… The hippocampal CA1 was cut with laser. Scale bar = 200 µm. Click to view full-size image… The hippocampal CA1 was removed by LCM. Scale bar = 200 µm. Click to view full-size image… The hippocampal CA1 was captured by LCM for total RNA extraction. Scale bar = 200 µm. This work is licensed under a Creative Commons Attribution 2.5 License.
Lab	CMMT: Elizabeth Simpson
Number of Animals	Not Available

Library Construction

SAGE Protocol	SAGELite (LongSAGE 21 bp)
Starting RNA	32.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off)	108,538
Total Raw Tags at Q95	78,388
Total Raw Tags at Q99	74,887
Unique Tags	35,402

Data

Library Metadata	Library Metadata XML
Tag Counts at Quality 0.95 cut-off	tagcounts_SM186_Q95.txt
Tag Counts at Quality 0.99 cut-off	tagcounts_SM186_Q99.txt
Clustered Tag Counts and P-Value	tagclustered_SM186_Q99.txt

Data Analysis

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.

Mouse Atlas of Gene Expression

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