SAGE Library SM186

Classification

Library Name SM186
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - CA1, dorsal/anterior
Sex Male
Age P35
Developmental Stage Post natal day 35
Organsim Condition

Sample Collection

Tissue Dissection Photos

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An intact coronal cryosection of mouse brain at postnatal day 35 stained with cresyl violet. Ctx = cerebral cortex; cc = corpus callosum; DG = dentate gyrus. Scale bar = 200 µm.


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The hippocampal CA1 was cut with laser. Scale bar = 200 µm.


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The hippocampal CA1 was removed by LCM. Scale bar = 200 µm.


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The hippocampal CA1 was captured by LCM for total RNA extraction. Scale bar = 200 µm.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals Not Available

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 32.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 108,538
Total Raw Tags at Q95 78,388
Total Raw Tags at Q99 74,887
Unique Tags 35,402

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM186_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM186_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM186_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.