SAGE Library SM181

Classification

Library Name SM181
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - Rahpe nuclei
Sex Male
Age P84-P90
Developmental Stage 12 weeks post birth
Organsim Condition

Sample Collection

Tissue Dissection Photos

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An intact coronal cryosection of mouse brain at postnatal week 12 stained with cresyl violet. Aq = aquaduct; RN = raphe nuclei; InCo = intercollicular nucleus; DpMe = deep mesencephalic nucleus; MnR = median raphe nucleus. Scale bar = 200 µm.


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The raphe nuclei was cut with laser. Scale bar = 200 µm.


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The raphe nuclei was removed by LCM. Scale bar = 200 µm.


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The raphe nuclei was captured by LCM for total RNA extraction. Scale bar = 200 µm.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals Not Available

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 82.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 105,732
Total Raw Tags at Q95 85,075
Total Raw Tags at Q99 79,509
Unique Tags 30,622

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM181_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM181_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM181_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.