SAGE Library SM180

Classification

Library Name SM180
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - Locus coeruleus
Sex Male
Age P84-P90
Developmental Stage 12 weeks post birth
Organsim Condition

Sample Collection

Tissue Dissection Photos

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An intact coronal cryosection of mouse brain at postnatal week 12 stained with cresyl violet. 4V = 4TH ventricle; LTDg = lateral dorsal tegmental nucleus; LC = locus coeruleus. Scale bar = 200 µm.


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The locus coeruleus was cut with laser. Scale bar = 200 µm.


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The locus coeruleus was removed by LCM. Scale bar = 200 µm.


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The locus coeruleus was captured by LCM for total RNA extraction. Scale bar = 200 µm.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals Not Available

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 100.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 102,956
Total Raw Tags at Q95 77,457
Total Raw Tags at Q99 73,434
Unique Tags 33,576

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM180_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM180_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM180_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.