SAGE Library SM166

Classification

Library Name SM166
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Pituitary gland - Whole
Sex Male
Age P0
Developmental Stage Theiler Stage 27
Organsim Condition

Sample Collection

Tissue Dissection Photos

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Brain cavity showing pituitary gland.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab BCCRC: Pamela Hoodless Lab
Number of Animals Not Available

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 200.0 ng

Tag Extraction

SM165 and SM166 were constructed using the same RNA source, with 2500 ng and 200 ng DNase I treated RNA respectively, ditags were amplified for 25 and 29 cycles respectively, tags were extracted and correlated.

Merged Library Name SM165_SM166
Total Raw Tags (no quality cut-off) 116,705
Total Raw Tags at Q95 77,523
Total Raw Tags at Q99 74,472
Unique Tags 33,103

Data

Tag Counts at Quality 0.95 cut-off tagcounts-896_SM165_SM166_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts-896_SM165_SM166_Q99.txt
Clustered Tag Counts and P-Value tagclustered-896_SM165_SM166.txt
Original Tag Set
Total Raw Tags (no quality cut-off) 60,980
Total Raw Tags at Q95 49,642
Total Raw Tags at Q99 47,437
Unique Tags 19,767

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM166_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM166_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM166.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.