SAGE Library SM166

Classification

Library Name	SM166
Species	Mus musculus
Strain	C57BL/6J
Mutation	Wild Type
Location	Pituitary gland - Whole
Sex	Male
Age	P0
Developmental Stage	Theiler Stage 27
Organsim Condition

Sample Collection

Tissue Dissection Photos	Click to view full-size image… Click to view full-size image… Brain cavity showing pituitary gland. This work is licensed under a Creative Commons Attribution 2.5 License.
Lab	BCCRC: Pamela Hoodless Lab
Number of Animals	Not Available

Library Construction

SAGE Protocol	SAGE (LongSAGE 21 bp)
Starting RNA	200.0 ng

Tag Extraction

SM165 and SM166 were constructed using the same RNA source, with 2500 ng and 200 ng DNase I treated RNA respectively, ditags were amplified for 25 and 29 cycles respectively, tags were extracted and correlated.

Merged Library Name	SM165_SM166
Total Raw Tags (no quality cut-off)	116,705
Total Raw Tags at Q95	77,523
Total Raw Tags at Q99	74,472
Unique Tags	33,103

Data

Tag Counts at Quality 0.95 cut-off	tagcounts-896_SM165_SM166_Q95.txt
Tag Counts at Quality 0.99 cut-off	tagcounts-896_SM165_SM166_Q99.txt
Clustered Tag Counts and P-Value	tagclustered-896_SM165_SM166.txt

Original Tag Set

Total Raw Tags (no quality cut-off)	60,980
Total Raw Tags at Q95	49,642
Total Raw Tags at Q99	47,437
Unique Tags	19,767

Data

Library Metadata	Library Metadata XML
Tag Counts at Quality 0.95 cut-off	tagcounts_SM166_Q95.txt
Tag Counts at Quality 0.99 cut-off	tagcounts_SM166_Q99.txt
Clustered Tag Counts and P-Value	tagclustered_SM166.txt

Data Analysis

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.

Mouse Atlas of Gene Expression

Sections

SAGE Library SM166

Classification

Sample Collection

Library Construction

Tag Extraction

Data

Data

Data Analysis

SAGE Library Help