SAGE Library SM148

Classification

Library Name	SM148
Species	Mus musculus
Strain	C57BL/6J
Mutation	Wild Type
Location	Brain - Striatum
Sex	Unknown
Age	E17.5
Developmental Stage	Theiler Stage 25
Organsim Condition

Sample Collection

Tissue Dissection Photos	Click to view full-size image… An intact coronal cryosection of mouse brain at embryonic day 17.5 stained with cresyl violet. MZ, marginal zone; CP, cortical plate; IZ, intermediate zone; VZ/SVZ, ventricular/subventricular zone; Str., striatum; IC, internal capsule; V, lateral ventricle. Scale bar = 200 µm. Click to view full-size image… The striatum was cut with laser. Scale bar = 200 µm. Click to view full-size image… The striatum was removed by LCM. Scale bar = 200 µm. Click to view full-size image… The striatum was captured by LCM for total RNA extraction. Scale bar = 200 µm. This work is licensed under a Creative Commons Attribution 2.5 License.
Lab	CMMT: Elizabeth Simpson
Number of Animals	Not Available

Library Construction

SAGE Protocol	SAGELite (LongSAGE 21 bp)
Starting RNA	72.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off)	121,321
Total Raw Tags at Q95	96,332
Total Raw Tags at Q99	91,753
Unique Tags	33,642

Data

Library Metadata	Library Metadata XML
Tag Counts at Quality 0.95 cut-off	tagcounts_SM148_Q95.txt
Tag Counts at Quality 0.99 cut-off	tagcounts_SM148_Q99.txt
Clustered Tag Counts and P-Value	tagclustered_SM148_Q99.txt

Data Analysis

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.

Mouse Atlas of Gene Expression

Sections