SAGE Library SM137

Classification

Library Name SM137
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - Dentate gyrus, dorsal/anterior
Sex Male
Age P84-P90
Developmental Stage 12 weeks post birth
Organsim Condition

Sample Collection

Tissue Dissection Photos

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An intact, 20 µm thick, coronal cryosection of 12 week old male mouse stained with cresyl violet. Approximately Bregma – 1.35. DG = dentate gyrus; D3V = dorsal third ventricle; D = dorsal; V = ventral. Scale bar = 100 µm


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A 20 µm thick, cresyl violet stained, coronal cryosection of 12 week old male mouse after DG removed by laser capture microdissection (LCM). Approximately Bregma – 1.35. DG = dentate gyrus; D3V = dorsal third ventricle; D = dorsal; V = ventral. Scale bar = 100 µm.


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3. An intact, 20 µm thick, coronal cryosection of 12 week old male mouse. Intact Dentate Gyrus of the left hemisphere, stained with cresyl violet. DG = dentate gyrus; D = dorsal; V = ventral. Scale bar = 100 µm.


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Dentate gyrus cut out by aid of laser. Thin regions (tips of blades) were avoided to minimize burning of tissue and therefore maintain high RNA quality. DG = dentate gyrus; D = dorsal; V = ventral. Scale bar = 100 µm.


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Dentate gyrus has been removed from brain section by means of LCM. DG = dentate gyrus; D = dorsal; V = ventral. Scale bar = 100 µm.


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Laser captured dentate gyrus on cap. DG = dentate gyrus; Scale bar = 100 µm.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals 3

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 27.65 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 104,336
Total Raw Tags at Q95 77,739
Total Raw Tags at Q99 74,728
Unique Tags 30,441

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM137_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM137_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM137_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.