SAGE Library SM122

Classification

Library Name SM122
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - Whole head
Sex Male
Age E9.5
Developmental Stage Theiler Stage 15
Organsim Condition

Sample Collection

Tissue Dissection Photos

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Sagittal view of dissected whole head, cut off just below otic vesicle, encompasses forebrain, midbrain and hindbrain – R side visible


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Sagittal view of dissected whole head, cut off just below otic vesicle, encompasses forebrain, midbrain and hindbrain – L side visible

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals Not Available

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 100.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 107,642
Total Raw Tags at Q95 88,523
Total Raw Tags at Q99 84,828
Unique Tags 28,208

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM122_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM122_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM122_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.