SAGE Library SM120

Classification

Library Name SM120
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Skin
Sex Unknown
Age E13.5
Developmental Stage Theiler Stage 21
Organsim Condition

Sample Collection

Lab BCCRC: Cheryl Helgason Lab
Number of Animals Not Available

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 500.0 ng

Tag Extraction

SM119 and SM120 were constructed using the same RNA source, with 5000 ng and 500 ng DNase I treated RNA respectively, ditags were amplified for 25 and 27 cycles respectively, tags were extracted and correlated.

Merged Library Name SM119_SM120
Total Raw Tags (no quality cut-off) 102,088
Total Raw Tags at Q95 76,507
Total Raw Tags at Q99 73,406
Unique Tags 35,786

Data

Tag Counts at Quality 0.95 cut-off tagcounts-901_SM119_SM120_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts-901_SM119_SM120_Q99.txt
Clustered Tag Counts and P-Value tagclustered-901_SM119_SM120.txt
Original Tag Set
Total Raw Tags (no quality cut-off) 50,860
Total Raw Tags at Q95 39,977
Total Raw Tags at Q99 38,111
Unique Tags 21,497

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM120_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM120_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM120.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

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