SAGE Library SM117

Classification

Library Name SM117
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Neural tube - Posterior neuropore
Sex Unknown
Age E8.5
Developmental Stage Theiler Stage 13
Organsim Condition

Sample Collection

Tissue Dissection Photos

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With the aid of a dissecting microscope, the extraembryonic tissue was manually removed from embryos in phosphate buffered saline (PBS). To dissociate the germ layers, embryos were subsequently placed in 500 uL of 1% trypsin in Hanks solution. After 30 minutes of incubation on ice, the digestion was stopped with the addition of 1 mL of 20% fetal bovine serum (FBS) in Hanks solution plus a small amount of DNase I powder. Tungsten needles or fine forceps were used to tease away the whole neural ectoderm from other two germ layers. Then the posterior neural pore was removed and collected in tubes containing Trizol.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab BCCRC: Pamela Hoodless Lab
Number of Animals 100

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 2.5 ug

Tag Extraction

Total Raw Tags (no quality cut-off) 102,336
Total Raw Tags at Q95 78,376
Total Raw Tags at Q99 74,203
Unique Tags 33,282

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM117_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM117_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM117_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

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