SAGE Library SM111


Library Name SM111
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - Whole
Sex Male
Age E17.5
Developmental Stage Theiler Stage 25
Organsim Condition

Sample Collection

Tissue Dissection Photos

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Mid-dissection – embryo decapitated and brain exposed as skin and skull peeled away.

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Dissected whole brain – may contain some upper spinal cord.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals 1

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 100.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 102,866
Total Raw Tags at Q95 80,048
Total Raw Tags at Q99 75,791
Unique Tags 42,220


Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM111_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM111_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM111_Q99.txt

Data Analysis


Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.