SAGE Library SM110

Classification

Library Name SM110
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - Hypothalamus
Sex Male
Age P84
Developmental Stage 12 weeks post birth
Organsim Condition

Sample Collection

Tissue Dissection Photos

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An intact coronal cryosection of mouse brain at postnatal week 12 stained with cresyl violet. ic = internal capsule; VT = ventral thalamus; LH = lateral hypothalamus; H = hypothalmus; 3V = 3RD ventricle. Scale bar = 200 µm.


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The hypothalamus was cut with laser. Scale bar = 200 µm.


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The hypothalamus was removed by LCM. Scale bar = 200 µm.


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The hypothalamus was captured by LCM for total RNA extraction. Scale bar = 200 µm.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals 1

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 114.5 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 108,936
Total Raw Tags at Q95 84,996
Total Raw Tags at Q99 80,798
Unique Tags 34,407

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM110_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM110_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM110_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.