SAGE Library SM099

Classification

Library Name SM099
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Embryo - Extraembryonic
Sex Unknown
Age E7.5
Developmental Stage Theiler Stage 11
Organsim Condition

Sample Collection

Tissue Dissection Photos

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With the aid of a dissecting microscope deciduas were teased apart under phosphate buffered saline (PBS) to expose embryos. The embryonic region (as defined in the organogenic image) was severed from the rest of the embryos using either mini-Vannas scissors or fine forceps. The tissue was then collected into Trizol. The embryonic tissue of several litters was collected to obtain enough RNA for SAGE library construction.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab BCCRC: Pamela Hoodless Lab
Number of Animals 100

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 8.25 ug

Tag Extraction

Total Raw Tags (no quality cut-off) 115,957
Total Raw Tags at Q95 88,726
Total Raw Tags at Q99 83,129
Unique Tags 33,176

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM099_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM099_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM099_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.