SAGE Library SM090

Classification

Library Name SM090
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Embryo - Embryonic
Sex Unknown
Age E7.5
Developmental Stage Theiler Stage 11
Organsim Condition

Sample Collection

Tissue Dissection Photos

Click to view full-size image…

With the aid of a dissecting microscope deciduas were teased apart under phosphate buffered saline (PBS) to expose embryos. The extraembryonic region (as defined in the organogenic image) was severed from the rest of the embryos using either mini-Vannas scissors or fine forceps. The tissue was then collected into Trizol. The extraembryonic tissue of several litters was collected to obtain enough RNA for SAGE library construction.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab BCCRC: Pamela Hoodless Lab
Number of Animals 100

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 9.99 ug

Tag Extraction

Total Raw Tags (no quality cut-off) 111,122
Total Raw Tags at Q95 88,875
Total Raw Tags at Q99 84,333
Unique Tags 29,623

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM090_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM090_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM090_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.