SAGE Library SM088

Classification

Library Name SM088
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - Hypothalamus
Sex Unknown
Age E12.5
Developmental Stage Theiler Stage 20
Organsim Condition

Sample Collection

Tissue Dissection Photos

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An intact sagittal brain section of mouse at E12.5 stained with cresyl violet. G.E. = ganglionic eminence; E. = epithalamus; D.T. = dorsal thalamus; H. = hypothalmus; O.R. = optic recess. Scale bar = 100 µm.


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Hypothalamus of diencephalon was cut by laser (arrow). Scale bar = 100 µm.


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Hypothalamus of diencephalon was removed by LCM. Scale bar = 100 µm.


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Hypothalamus of diencephalon was captured by LCM. Scale bar = 100 µm.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals 2

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 54.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 132,890
Total Raw Tags at Q95 106,323
Total Raw Tags at Q99 100,576
Unique Tags 35,805

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM088_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM088_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM088_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.