SAGE Library SM087

Classification

Library Name SM087
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - Diencephalon
Sex Unknown
Age E11.5
Developmental Stage Theiler Stage 19
Organsim Condition

Sample Collection

Tissue Dissection Photos

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An intact sagittal cryosection of mouse brain at embryonic day (E) 11.5 was stained with cresyl violet. Scale bar = 100 µm.


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The Diencephalon (arrow) was outlined and cut by laser. Scale bar = 180 µm.


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The Diencephalon (arrow) was removed by LCM. Scale bar = 100 µm.


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The Diencephalon was captured by LCM. Scale bar = 180 µm.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals 2

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 90.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 113,907
Total Raw Tags at Q95 90,099
Total Raw Tags at Q99 85,713
Unique Tags 32,069

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM087_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM087_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM087_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.