SAGE Library SM083

Classification

Library Name SM083
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - Future forebrain
Sex Unknown
Age E8.5
Developmental Stage Theiler Stage 13
Organsim Condition

Sample Collection

Tissue Dissection Photos

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E8.5 C57BL/6J embryo just before turning, viewed from the top (back). 1. Microscope focused on somite pairs. 2. Microscope focused on developing brain (mid and hind region).


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Forebrain region to be cut out is outlined in blue. Note that there are two lobes which have not yet fused completely (denoted by dark and light blue dashed lines). Top and bottom labels correspond to view of dissected tissue in the following image.


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Dissected E8.5 future forebrain tissue. 1. As would be seen looking up from underside of embryo. 2. As seen from top of embryo.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals 5

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 200.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 120,113
Total Raw Tags at Q95 94,702
Total Raw Tags at Q99 90,282
Unique Tags 34,000

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM083_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM083_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM083_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.