SAGE Library SM081

Classification

Library Name SM081
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - Whole head
Sex Unknown
Age E8.5
Developmental Stage Theiler Stage 13
Organsim Condition

Sample Collection

Tissue Dissection Photos

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Whole E8.5 embryo still in amnion.


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E8.5 future brain region to be dissected is outlined in blue. It includes future forebrain (telencephalon, diencephalon), midbrain (mesencephalon), and hindbrain (metencephalon, rhombencephalon).


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Manually dissected whole future brain from E8.5 C57BL/6J embryo (future forebrain, midbrain, and hindbrain).

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals 2

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 300.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 110,057
Total Raw Tags at Q95 89,319
Total Raw Tags at Q99 85,312
Unique Tags 30,386

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM081_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM081_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM081_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.