SAGE Library SM077

Classification

Library Name SM077
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Embryo - Fertilized eggs
Sex Unknown
Age E0.5
Developmental Stage 1 Cell - Theiler Stage 1
Organsim Condition

Sample Collection

Tissue Dissection Photos

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All embryos were collected from superovulated females. The superovulation procedure was performed on 6-week old C57BL/6J females as described in Nagy et al., 2003 (Manipulating the Mouse Embryo). The collection of fertilized eggs and removal of cumulus cells with hyaluronidase was conducted as described by Nagy et al. (2003). Fertilized eggs were then transferred to Trizol. Multiple litters were used to collect enough RNA for SAGE library construction.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab BCCRC: Pamela Hoodless Lab
Number of Animals 426

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 75.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 111,136
Total Raw Tags at Q95 90,643
Total Raw Tags at Q99 86,155
Unique Tags 40,491

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM077_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM077_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM077_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

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