SAGE Library SM072

Classification

Library Name SM072
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Stomach - Whole
Sex Unknown
Age E12
Developmental Stage Theiler Stage 20
Organsim Condition

Sample Collection

Tissue Dissection Photos

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With the aid of a dissecting microscope the stomach was manually removed from embryos submerged in phosphate buffered saline (PBS). The isolated stomachs were then transferred to Trizol. The stomachs of embryos of several litters were pooled together to obtain enough RNA for SAGE library construction

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab BCCRC: Pamela Hoodless Lab
Number of Animals 68

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 10.0 ug

Tag Extraction

Total Raw Tags (no quality cut-off) 108,246
Total Raw Tags at Q95 91,903
Total Raw Tags at Q99 86,056
Unique Tags 31,557

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM072_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM072_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM072_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.