SAGE Library SM070

Classification

Library Name SM070
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - Whole telencephalon
Sex Unknown
Age E9.5
Developmental Stage Theiler Stage 15
Organsim Condition

Sample Collection

Tissue Dissection Photos

Click to view full-size image…

An intact sagittal cryosection of mouse embryo at E9.5 (TS 15) stained with cresyl violet. Scale bar = 200 µm.


Click to view full-size image…

Telencephalon was cut by laser (arrows). Scale bar = 200 µm.


Click to view full-size image…

Telencephalon was removed by LCM. Scale bar = 200 µm.


Click to view full-size image…

Telencephalon was captured by LCM. Scale bar = 200 µm.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals 12

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 50.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 117,979
Total Raw Tags at Q95 93,864
Total Raw Tags at Q99 89,381
Unique Tags 30,683

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM070_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM070_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM070_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.