SAGE Library SM067

Classification

Library Name SM067
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - Dorsal telencephalon
Sex Unknown
Age E10.5
Developmental Stage Theiler Stage 17
Organsim Condition

Sample Collection

Tissue Dissection Photos

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An intact sagittal cryosection of embryonic brain of mouse at E10.5 (TS 17) stained with cresyl violet. Scale bar = 200 µm.


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The ventricular and subventricular zone (VZ/SVZ) of the dorsal telencephalon was cut by laser (arrows). Scale bar = 200 µm.


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The VZ/SVZ of the dorsal telencephalon was removed by LCM. Scale bar = 200 µm.


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The VZ/SVZ of the dorsal telencephalon was captured by LCM. Scale bar = 200 µm.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals 5

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 14.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 103,249
Total Raw Tags at Q95 81,347
Total Raw Tags at Q99 77,035
Unique Tags 27,547

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM067_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM067_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM067_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.