SAGE Library SM064

Classification

Library Name	SM064
Species	Mus musculus
Strain	C57BL/6J
Mutation	Wild Type
Location	Brain - Prestriatum
Sex	Unknown
Age	E12.5
Developmental Stage	Theiler Stage 20
Organsim Condition

Sample Collection

Tissue Dissection Photos	Click to view full-size image… An intact sagittal cryosection of telencephalon at embryonic day (E) 12.5 was stained with cresyl violet. LV = lateral ventricle; D = dorsal; V = ventral; R = rostral. Scale bar = 180 µm. Click to view full-size image… The prestriatum (arrows) was cut by laser with laser capture microdissection (LCM). Scale bar = 180 µm. Click to view full-size image… The prestriatum region was removed by LCM. Scale bar = 180 µm. Click to view full-size image… The prestriatum region was captured by LCM. Scale bar = 180 µm. This work is licensed under a Creative Commons Attribution 2.5 License.
Lab	CMMT: Elizabeth Simpson
Number of Animals	1

Library Construction

SAGE Protocol	SAGELite (LongSAGE 21 bp)
Starting RNA	10.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off)	147,906
Total Raw Tags at Q95	130,721
Total Raw Tags at Q99	124,411
Unique Tags	34,213

Data

Library Metadata	Library Metadata XML
Tag Counts at Quality 0.95 cut-off	tagcounts_SM064_Q95.txt
Tag Counts at Quality 0.99 cut-off	tagcounts_SM064_Q99.txt
Clustered Tag Counts and P-Value	tagclustered_SM064_Q99.txt

Data Analysis

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.

Mouse Atlas of Gene Expression

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