SAGE Library SM060

Classification

Library Name SM060
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - Visual cortex
Sex Male
Age P84-P90
Developmental Stage 12 weeks post birth
Organsim Condition

Sample Collection

Tissue Dissection Photos

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Schematic of visual cortex dissection from: Gordon, J.A. and M. P. Stryker (1996). “Experience-dependent plasticity of binocular responses in the primary visual cortex of the mouse.” J Neurosci 16(10): 3274-86


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12 week male C57BL/6J mouse. Brain uncut.


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12 week male C57BL/6J mouse. Visual cortex region cut out from both hemispheres. Cut out tissue from L hemisphere resting on scalpel.


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12 week male C57BL/6J mouse. 1 & 2. Visual cortex region cut out from both hemispheres. 3. Cut out tissue from L hemisphere resting on scalpel.


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12 week male C57BL/6J mouse. Visual cortex region cut out from both hemispheres.


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12 week male C57BL/6J mouse. 1 & 2. Visual cortex region cut out from both hemispheres.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals 3

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 5.0 ug

Tag Extraction

Total Raw Tags (no quality cut-off) 124,900
Total Raw Tags at Q95 103,974
Total Raw Tags at Q99 98,637
Unique Tags 40,387

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM060_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM060_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM060_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.