SAGE Library SM053

Classification

Library Name SM053
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - VZ/SVZ dorsal telencephalon
Sex Unknown
Age E12.5
Developmental Stage Theiler Stage 20
Organsim Condition

Sample Collection

Tissue Dissection Photos

Click to view full-size image…

An intact sagittal cryosection of telencephalon at embryonic day (E) 12.5 was stained with cresyl violet. SVZ/VZ = subventricular and ventricular zone; LV = lateral ventricle; D = dorsal; V = ventral; R = rostral. Scale bar = 100 µm.


Click to view full-size image…

The SVZ/VZ of dorsal telencephalon (arrow) was cut by laser with laser capture microdissection (LCM). Scale bar = 180 µm.


Click to view full-size image…

The SVZ/VZ of dorsal telencephalon (arrow) was removed by LCM. Scale bar = 100 µm.


Click to view full-size image…

The SVZ/VZ of dorsal telencephalon (arrow) was captured by LCM. Scale bar = 180 µm.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals 1

Library Construction

SAGE Protocol SAGELite (LongSAGE 21 bp)
Starting RNA 95.0 ng

Tag Extraction

Total Raw Tags (no quality cut-off) 117,767
Total Raw Tags at Q95 98,229
Total Raw Tags at Q99 92,997
Unique Tags 27,145

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM053_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM053_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM053_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.