SAGE Library SM051


Library Name SM051
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Heart - Ventricle
Sex Unknown
Age E11.5
Developmental Stage Theiler Stage 19
Organsim Condition

Sample Collection

Tissue Dissection Photos

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With the aid of a dissecting microscope hearts were manually removed from embryos submerged in phosphate buffered saline (PBS). The ventricles were isolated using mini-Vannas scissors or fine forceps. The ventricular region is shown in the organogenic image. The outflow tract was removed from the ventricular tissue. As much blood was removed as possible from the ventricles before transfer to Trizol. The ventricles of embryos of several litters were collected to obtain enough RNA for SAGE library construction.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab BCCRC: Pamela Hoodless Lab
Number of Animals 38

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 11.1 ug

Tag Extraction

Total Raw Tags (no quality cut-off) 133,781
Total Raw Tags at Q95 115,264
Total Raw Tags at Q99 109,689
Unique Tags 34,330


Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM051_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM051_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM051_Q99.txt

Data Analysis


Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

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