SAGE Library SM042


Library Name SM042
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Muscle - Skeletal-gastrocnemius
Sex Unknown
Age E17
Developmental Stage Theiler Stage 25
Organsim Condition

Sample Collection

Tissue Dissection Photos

Click to view full-size image…

Both left and right gastrocnemius (calf) muscles were manually dissected. Tissue preperations are primarily gastrocnemius but will contain some other proximal muscle tissue and adhering mesenchyme. An effort was made to avoid including any fragments of bone.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab BCCRC: Pamela Hoodless Lab
Number of Animals Not Available

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 5.0 ug

Tag Extraction

Total Raw Tags (no quality cut-off) 137,201
Total Raw Tags at Q95 115,712
Total Raw Tags at Q99 107,009
Unique Tags 34,783


Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM042_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM042_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM042_Q99.txt

Data Analysis


Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.