SAGE Library SM031

Classification

Library Name SM031
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Brain - Visual cortex
Sex Male
Age P20
Developmental Stage Post natal day 20
Organsim Condition

Sample Collection

Tissue Dissection Photos

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Schematic of visual cortex dissection from: Gordon, J.A. and M.P. Stryker (1996) "Experience dependent plasticity of binocular responses in the primary visual cortex of the mouse" J Neusosci 16(10): 3274-86


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Visual Cortex cut out from right hemisphere; dissected tissue present.


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Right side visual cortex cut out; dissected tissue present (outlined by light blue dashes).


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Visual cortex region cut out from both hemispheres. Image shows white matter remaining underneath in the depression after dissection.


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Visual cortex dissected from both brain hemispheres. Blue dashes outline removed region.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab CMMT: Elizabeth Simpson
Number of Animals 10

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 5.0 ug

Tag Extraction

Total Raw Tags (no quality cut-off) 152,702
Total Raw Tags at Q95 110,971
Total Raw Tags at Q99 102,711
Unique Tags 46,799

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM031_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM031_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM031_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.