SAGE Library SM008

Same tissue sample as SM241.

Classification

Library Name SM008
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Heart - Atrioventricular canal
Sex Unknown
Age E11.5
Developmental Stage Theiler Stage 19
Organsim Condition

Sample Collection

Tissue Dissection Photos

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With the aid of a dissecting microscope hearts were manually removed from embryos submerged in phosphate buffered saline (PBS). The atrio-ventricular canal region was isolated using mini-Vannas scissors or fine forceps. The region designated atrio-ventricular canal is shown in the organogenic image. As much blood was removed as possible from the atria before transfer to Trizol. The atrio-ventricular canals of embryos of several litters were collected to obtain enough RNA for SAGE library construction.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab BCCRC: Pamela Hoodless Lab
Number of Animals 48

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 6.3 ug

Tag Extraction

Total Raw Tags (no quality cut-off) 117,715
Total Raw Tags at Q95 74,276
Total Raw Tags at Q99 66,768
Unique Tags 36,631

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM008_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM008_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM008_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.