SAGE Library SM007

Classification

Library Name SM007
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Heart - Left ventricle
Sex Unknown
Age E9.5
Developmental Stage Theiler Stage 14
Organsim Condition

Sample Collection

Tissue Dissection Photos

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With the aid of a dissecting microscope hearts were manually removed from embryos submerged in phosphate buffered saline (PBS). The primitive ventricular region was isolated using mini-Vannas scissors or fine forceps and then transferred to Trizol. The region designated primitive ventricle is shown in the organogenic image. The primitive ventricular region from embryos of several litters was collected to obtain enough RNA for SAGE library construction

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab BCCRC: Pamela Hoodless Lab
Number of Animals 78

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 4.7 ug

Tag Extraction

Total Raw Tags (no quality cut-off) 126,565
Total Raw Tags at Q95 73,865
Total Raw Tags at Q99 65,321
Unique Tags 37,009

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM007_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM007_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM007_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.