SAGE Library SM005

Classification

Library Name SM005
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Heart - Bulbus cordis
Sex Unknown
Age E9.5
Developmental Stage Theiler Stage 14
Organsim Condition

Sample Collection

Tissue Dissection Photos

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With the aid of a dissecting microscope hearts were manually removed from embryos submerged in phosphate buffered saline (PBS). Bulbus cordis regions were isolated using mini-Vannas scissors or fine forceps and then transferred to Trizol. The region designated bulbus cordis is shown in the organogenic image. The bulbus cordis region from embryos of several litters was collected to obtain enough RNA for SAGE library construction

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab BCCRC: Pamela Hoodless Lab
Number of Animals 79

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 5.0 ug

Tag Extraction

Total Raw Tags (no quality cut-off) 107,262
Total Raw Tags at Q95 81,812
Total Raw Tags at Q99 77,222
Unique Tags 30,012

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM005_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM005_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM005_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.