SAGE Library SM004

Classification

Library Name SM004
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Heart - Atria
Sex Unknown
Age E11.5
Developmental Stage Theiler Stage 19
Organsim Condition

Sample Collection

Tissue Dissection Photos

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With the aid of a dissecting microscope hearts were manually removed from embryos submerged in phosphate buffered saline (PBS). The atria were isolated using mini-Vannas scissors or fine forceps. The atria region is as shown in the organogenic image. As much blood was removed as possible from the atria before transfer to Trizol. The atria of embryos of several litters were collected to obtain enough RNA for SAGE library construction.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab BCCRC: Pamela Hoodless Lab
Number of Animals 48

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 5.0 ug

Tag Extraction

Total Raw Tags (no quality cut-off) 108,637
Total Raw Tags at Q95 66,817
Total Raw Tags at Q99 60,695
Unique Tags 28,471

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM004_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM004_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM004_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.