SAGE Library SM002

Classification

Library Name SM002
Species Mus musculus
Strain C57BL/6J
Mutation Wild Type
Location Heart - Atria
Sex Unknown
Age E12
Developmental Stage Theiler Stage 20
Organsim Condition

Sample Collection

Tissue Dissection Photos

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With the aid of a dissecting microscope hearts were manually removed from embryos submerged in phosphate buffered saline (PBS). The atria were isolated using mini-Vannas scissors or fine forceps. The region designated atria is shown in the organogenic image. As much blood was removed as possible from the atria before transfer to Trizol. The atria from 34 embryos of several liters was collected to obtain enough RNA for SAGE library construction.

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.
Lab BCCRC: Pamela Hoodless Lab
Number of Animals 34

Library Construction

SAGE Protocol SAGE (LongSAGE 21 bp)
Starting RNA 8.95 ug

Tag Extraction

Total Raw Tags (no quality cut-off) 99,271
Total Raw Tags at Q95 55,216
Total Raw Tags at Q99 49,927
Unique Tags 23,862

Data

Library Metadata Library Metadata XML
Tag Counts at Quality 0.95 cut-off tagcounts_SM002_Q95.txt
Tag Counts at Quality 0.99 cut-off tagcounts_SM002_Q99.txt
Clustered Tag Counts and P-Value tagclustered_SM002_Q99.txt

Data Analysis

DiscoverySpace

Data is available for analysis using DiscoverySpace, a cross-platform application that allows comparison between SAGE libraries, tag to gene mapping and results from public datasources.

SAGE Library Help

A full explanation of each of the fields displayed on this page is available on the SAGE Library Help page.